Sustainable international experience: A collaborative teaching project

Within engineering education, there is an increasing need for providing our students with international experiences. This is most often done by exchange studies abroad. However, a majority of the students on engineering programs do not engage in any international exchange. This paper presents insights from a collaborative cross-disciplinary international project to give students international experience without having to travel. From both a sustainability perspective and a situation where e.g. a global virus outbreak stop students from travelling, solutions that give engineering students experience of working in an international setting are becoming increasingly important. Initial challenges, for the teachers involved in the project, that were addressed before the project started, included the assessment of students, the use of online collaborative tools, assessment of students and the dependence between the two courses. The learnings from the first and second iteration of the collaborative project were mainly focused around transparency, introduction of students to each other, communication, real-time issues and deadlines. By gradually remove these peripheral challenges for the students, resulting in making the students focus on the actual challenges surrounding the actual collaborative project. Even though this project is ongoing, the initial results clearly show that by integrating courses between different countries and disciplines, it is possible to create an environment that strengthens the students’ ability in teamwork, communication and addresses the cultural and professional aspects of working as an engineer in an international context

Internationalization of students’ learning using online technology: Lessons learned

The global nature of business has increased the importance of students’ international experience during their studies at the university. Using interactive technologies the projects investigated ways to increase students’ motivation to take responsibility for the learning process by creating “real” international co-creation experience online. This paper presents learnings from two consecutive international collaborative teaching between Edith Cowan University, Australia, and Umeå University, Sweden, in 2017 and Edith Cowan University and the University of Rijeka, Croatia, in 2018. Feedback from the students showed they enjoyed working across cultures and academic discipline on simulated products and marketing campaigns. Issues raised included: the need to explicitly explain how all parts of project is going to work and how the students execute their role. Incorporation of a formal introduction process for the students in each location so all students have the same knowledge about each other. Furthermore, the provision of real-time opportunities to collaborate in lectures and the setting joint deadlines between the units are of importance. Despite some of the shortcomings of the project, it has provided a firm foundation for the refinement of ongoing collaborative teaching

Evidence for wastewaters as environments where mobile antibiotic resistance genes emerge

The emergence and spread of mobile antibiotic resistance genes (ARGs) in pathogens have become a serious threat to global health. Still little is known about where ARGs gain mobility in the first place. Here, we aimed to collect evidence indicating where such\ua0initial mobilization\ua0events of clinically relevant ARGs may have occurred. We found that the majority of previously identified origin species did not carry the mobilizing elements that likely enabled intracellular mobility of the ARGs, suggesting a necessary interplay between different bacteria. Analyses of a broad range of metagenomes revealed that wastewaters and wastewater-impacted environments had by far the highest abundance of both origin species and corresponding mobilizing elements. Most origin species were only occasionally detected in other environments. Co-occurrence of origin species and corresponding mobilizing elements were rare in human microbiota. Our results identify wastewaters and wastewater-impacted environments as plausible arenas for the initial mobilization of resistance genes

GEnView: a gene-centric, phylogeny-based comparative genomics pipeline for bacterial genomes and plasmids

Comparing genomic loci of a given bacterial gene across strains and species can provide insights into their evolution, including information on e.g. acquired mobility, the degree of conservation between different taxa or indications of horizontal gene transfer events. While thousands of bacterial genomes are available to date, there is no software that facilitates comparisons of individual gene loci for a large number of genomes. GEnView (Genetic Environment View) is a Python-based pipeline for the comparative analysis of gene-loci in a large number of bacterial genomes, providing users with automated, taxon-selective access to the >800.000 genomes and plasmids currently available in the NCBI Assembly and RefSeq databases, and is able to process local genomes that are not deposited at NCBI, enabling searches for genomic sequences and to analyze their genetic environments through the interactive visualization and extensive metadata files created by GEnView

New and interesting records of lichens from Pältsan (Mt Bealccan) in northernmost Sweden

Austroplaca subtiroliensis, Gyalidea lecideopsis, Phacographa protoparmeliae, Placynthium pulvinatum and Solorina octospora are reported new to Sweden. Several other rare or rarely collected lichens are also reported: Absconditella annexa, Arthrorhaphis vacillans, Farnoldia micropsis, Gyalidea polyspora, Ionaspis ventosa, Lecanora lecanoricola, L. leptacinella, Lecidea commaculans, Pachyascus lapponicus, Placidiopsis pseudocinerea, Schadonia fecunda, Scytinium aquale and Thelocarpon sphaerosporum

On de Sitter Vacua in Type IIA Orientifold Compactifications

This letter discusses the orientifold projection of the quantum corrections to type IIA strings compactified on rigid Calabi-Yau threefolds. It is shown that N=2 membrane instanton effects give a holomorphic contribution to the superpotential, while the perturbative corrections enter into the Kahler potential. At the level of the scalar potential the corrections to the Kahler potential give rise to a positive energy contribution similar to adding anti-D3-branes in the KKLT scenario. This provides a natural mechanism to lift an AdS vacuum to a meta-stable dS vacuum.Comment: 5 pages, no figures, minor changes, references adde

Z_2 x Z_2 Heterotic Orbifold Models of Non Factorisable Six Dimensional Toroidal Manifolds

We discuss heterotic strings on Z_2 x Z_2 orbifolds of non factorisable six-tori. Although the number of fixed tori is reduced as compared to the factorisable case, Wilson lines are still needed for the construction of three generation models. An essential new feature is the straightforward appearance of three generation models with one generation per twisted sector. We illustrate our general arguments for the occurrence of that property by an explicit example. Our findings give further support for the conjecture that four dimensional heterotic strings formulated at the free fermionic point are related to Z_2 x Z_2 orbifolds.Comment: 33 pages, LaTeX; discussion of modular invariance added in section four; added references; to be published in JHE

Large-scale characterization of the macrolide resistome reveals high diversity and several new pathogen-associated genes

Macrolides are broad-spectrum antibiotics used to treat a range of infections. Resistance to macrolides is often conferred by mobile resistance genes encoding Erm methyltransferases or Mph phosphotransferases. New erm and mph genes keep being discovered in clinical settings but their origins remain unknown, as is the type of macrolide resistance genes that will appear in the future. In this study, we used optimized hidden Markov models to characterize the macrolide resistome. Over 16 terabases of genomic and metagenomic data, representing a large taxonomic diversity (11 030 species) and diverse environments (1944 metagenomic samples), were searched for the presence of erm and mph genes. From this data, we predicted 28 340 macrolide resistance genes encoding 2892 unique protein sequences, which were clustered into 663 gene families (<70 % amino acid identity), of which 619 (94 %) were previously uncharacterized. This included six new resistance gene families, which were located on mobile genetic elements in pathogens. The function of ten predicted new resistance genes were experimentally validated in Escherichia coli using a growth assay. Among the ten tested genes, seven conferred increased resistance to erythromycin, with five genes additionally conferring increased resistance to azithromycin, showing that our models can be used to predict new functional resistance genes. Our analysis also showed that macrolide resistance genes have diverse origins and have transferred horizontally over large phylogenetic distances into human pathogens. This study expands the known macrolide resistome more than ten-fold, provides insights into its evolution, and demonstrates how computational screening can identify new resistance genes before they become a significant clinical problem

Comprehensive screening of genomic and metagenomic data reveals a large diversity of tetracycline resistance genes

Tetracyclines are broad-spectrum antibiotics used to prevent or treat a variety of bacterial infections. Resistance is often mediated through mobile resistance genes, which encode one of the three main mechanisms: active efflux, ribosomal target protection or enzymatic degradation. In the last few decades, a large number of new tetracycline-resistance genes have been discovered in clinical settings. These genes are hypothesized to originate from environmental and commensal bacteria, but the diversity of tetracycline-resistance determinants that have not yet been mobilized into pathogens is unknown. In this study, we aimed to characterize the potential tetracycline resistome by screening genomic and metagenomic data for novel resistance genes. By using probabilistic models, we predicted 1254 unique putative tetracycline resistance genes, representing 195 gene families (<70 % amino acid sequence identity), whereof 164 families had not been described previously. Out of 17 predicted genes selected for experimental verification, 7 induced a resistance phenotype in an Escherichia coli host. Several of the predicted genes were located on mobile genetic elements or in regions that indicated mobility, suggesting that they easily can be shared between bacteria. Furthermore, phylogenetic analysis indicated several events of horizontal gene transfer between bacterial phyla. Our results also suggested that acquired efflux pumps originate from proteobacterial species, while ribosomal protection genes have been mobilized from Firmicutes and Actinobacteria. This study significantly expands the knowledge of known and putatively novel tetracycline resistance genes, their mobility and evolutionary history. The study also provides insights into the unknown resistome and genes that may be encountered in clinical settings in the future